Neural Reprogramming: Methods and Protocols (Methods in Molecular Biology)

Description: This detailed book brings together a number of state-of-the-art protocols to generate different types of neural cells through the use of reprogramming technologies. Additionally, the volume explores different aspects of functional evaluation and applications of reprogrammed neural cells as well as in silico methods to aid reprogramming efforts. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Neural Reprogramming: Methods and Protocols provides ample experimental experience and guidance for anyone, be it experienced researcher or beginner, to generate, validate, and apply reprogrammed neural cells in their research. 1. Isolation and Neuronal Reprogramming of Mouse Embryonic Fibroblasts Juan M. Adrian-Segarra, Bettina Weigel, and Moritz Mall 2. Direct In Vitro Reprogramming of Astrocytes into Induced Neurons Nesrin Sharif, Filippo Calzolari, and Benedikt Berninger 3. Direct Cell Reprogramming of Mouse Fibroblast into Functional Astrocytes Using Lentiviral Overexpression of the Transcription Factors NFIA, NFIB, and SOX9 Boning Qiu, Ruben J. de Vries, and Massimiliano Caiazzo 4. Direct Reprogramming of Fibroblasts to Astrocytes Using Small Molecules E Tian, Mingzi Zhang, and Yanhong Shi 5. Bcl-2-Assisted Reprogramming of Mouse Astrocytes and Human Fibroblasts into Induced Neurons Amel Falco, Rocío Bartolomé-Cabrero, and Sergio Gascón 6. Direct Conversion of Human Fibroblasts to Induced Neurons Lucia Zhou-Yang, Sophie Eichhorner, Lukas Karbacher, Lena Böhnke, Larissa Traxler, and Jerome Mertens 7. Generation of Induced Dopaminergic Neurons from Human Fetal Fibroblasts Emilie M. Legault and Janelle Drouin-Ouellet 8. Direct Differentiation of Functional Neurons from Human Pluripotent Stem Cells (hPSCs) Ruiqi Hu, Xiaoting Zhu, and Nan Yang 9. Generation of Motor Neurons from Human ESCs/iPSCs Using Sendai Virus Vectors Keiko Imamura, Jitsutaro Kawaguchi, Tsugumine Shu, and Haruhisa Inoue 10. Transcription Factor Programming of Human Pluripotent Stem Cells to Functionally Mature Astrocytes for Monocultures and Cocultures with Neurons Ella Quist, Henrik Ahlenius, and Isaac Canals 11. Single Transcription Factor-Based Differentiation Allowing Fast and Efficient Oligodendrocyte Generation via SOX10 Overexpression Katrien Neyrinck and Juan A. García-León 12. Transcriptional Profiling During Neural Conversion Yohannes Afeworki, Hannah Wollenzien, and Michael S. Kareta 13. Functional Assessment of Direct Reprogrammed Neurons In Vitro and In Vivo Srisaiyini Kidnapillai and Daniella Rylander Ottosson 14. Neurotransmitter Release of Reprogrammed Cells Using Electrochemical Detection Methods Andreas Heuer 15. Combining Cell Fate Reprogramming and Protein Engineering to Study Transcription Factor Functions Juan M. Adrian-Segarra, Bettina Weigel, and Moritz Mall 16. CRISPR/Cas9-Genome Engineering in Human Pluripotent Stem Cells for Modeling of Neurological Disorders Isaac Canals and Henrik Ahlenius 17. Derivation of Adult Human Cortical Organotypic Slice Cultures for Co-Culture with Reprogrammed Neuronal Cells Giedre Kvist

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End Time: 2025-01-09T18:35:27.000Z

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