Germinal Centers: Methods and Protocols (Methods in Molecular Biology)

Description: This volume provides key methods and protocols from laboratories engaged in germinal centers (GC) research with the expectation of stimulating further research, and to aid scientists in the study of GC biology and pathology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Germinal Centers: Methods and Protocols aims to ensure successful results in the further study of this vital field. 1. Analysis of the Germinal Center Reaction in Tissue Sections David Dominguez-Sola and Giorgio Cattoretti 2. Detecting Gene Expression in Lymphoid Microenvironments by Laser Microdissection and Quantitative RT-PCR Yang Zhang, Laura Garcia-Ibanez, Geoffrey Brown, and Kai-Michael Toellner 3. Tracking Plasma Cell Differentiation in Living Mice with 2-Photon Microscopy Carolin Ulbricht, Randall L. Lindquist, Laura Tech, and Anja E. Hauser 4. Microanatomical Labeling of Germinal Center Structures for Flow Cytometry Using Photoactivation Johanne T. Jacobsen and Gabriel D. Victora 5. Fate Mapping and Transcript Profiling of Germinal Center Cells by Two-photon Photoconversion Imogen Moran and Tri Giang Phan 6. Intravital Microscopy of T-B Cell Interactions in Germinal Centers Changming Shih and Hai Qi 7. Identifying Follicular Regulatory T cells by Confocal Microscopy Vanderleyden I, Linterman MA 8. Cytokine Expression by T follicular Helper Cells Christoph Jandl, Claudia Loetsch, and Cecile King 9. Follicular Dendritic Cell Isolation and Loading of Immune Complexes Balthasar A. Heesters, Cornelis E. Van der Poel, and Michael C. Carroll 10. Isolation and Characterization of Mouse and Human Follicular Dendritic Cells Mohey Eldin M. El Shikh, Riham El Sayed, and Costantino Pitzalis 11. In-vitro-induced Germinal Center B Cell Culture System Kei Haniuda, Takuya Nojima and Daisuke Kitamura 12. CRISPR/Cas9 Mediated In Vitro Mutagenesis in GC-like B Cells Van Trung Chu, Robin Graf and Klaus Rajewsky 13. Germinal Center Formation with Retrovirally-transduced B Cells for Determining the Role of Specific Molecules In Vivo Rinako Nakagawa 14. Characterization of the B Cell Transcriptome by RNA-Binding Proteins using iCLIP Manuel D. Díaz-Muñoz, Elisa Monzón-Casanova, and Martin Turner 15. Flow-Cytometric Method Measuring B Cell Surface Immunoglobulin Avidity Davide Angeletti, Gregory M. Frank, and Jonathan W. Yewdell 16. Somatic Hypermutation and Affinity Maturation Analysis Using the 4-hydroxy-3-nitrophenyl-acetyl (NP) System Nicole Heise and Ulf Klein 17. Targeting Gene Function in Germinal Center B Cells: A Practical Approach Valentina Petrocelli and Stefano Casola 18. Development of Mouse Model Systems of Germinal Centre Lymphomas Eleni Kabrani and Sandrine Sander 19. The AID-Cre-ERT2 Model: A Tool for Monitoring B Cell Immune Responses and Generating Selective Hybridomas Simon Le Gallou, Takuya Nojima, Daisuke Kitamura, Jean-Claude Weill, and Claude-Agnès Reynaud 20. Determining the Origin of Human Germinal Center B Cell-derived MalignanciesMarc Seifert and Ralf Küppers 21. Tracking B-cell Repertoires and Clonal Histories in Normal and Malignant Lymphocytes Nicola J. Weston-Bell, Graeme Cowan, and Surinder S. Sahota 22. How to Simulate a Germinal Centre Philippe A. Robert, Ananya Rastogi, Sebastian Binder, and Michael Meyer-Hermann

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Location: Hillsdale, NSW

End Time: 2025-02-06T14:02:22.000Z

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